In vitro Evaluation of Medicinal Plants’ Crude Extract against Black rot (Xanthomonas campestris pv. Campestris) of Cabbage

Abstract

Application of synthetic chemicals is one o f the primary options for the control
of the black rot disease. However, the environmental hazards and economic
unfeasibility associated with them necessitate the search for relatively safe
natural products with the ability to show antibacterial effects. With this view, an
exper iment wa s conducted to evaluate the effect of plant extracts on black rot o f
cabbage, Xanthomona s c ampes tris pv. Campestris (XCC). XCC was isolated
f rom infected c abbage leaves. The isolated pathogen grown in pure culture was
maintained in Yeast Extract Dextrose Calcium Carbonate Aga r (YDC) at 4"C.
The in vitro test wa s conducted to evaluate four methanol plant extract for their
antibacterial effects against XCC using the paper disc technique. The minimum
inhibitory concentration o f methanol extracts o f Phytolacca, dode candra, Ruta
chalepensis, Oc imum lamiifolium and Vernonia aniygdalina were 1.5. 2, 3 and
5%. respectively. In the in vitro trial, berries extracts o f P. dode candra at 10%
concentra tion (10.88mm) showed the highest growth inhibitory effects as
compa red to other plant extracts, followed by R. chalepensis at 10%
concentra tion (8.52mm) . In general, this study demons tra ted that plant extracts
such as P. dodecandra, R. chalepensis, O. lamiifolium and V. aniygdalina,
inhibits the growth o f XCC.