Necrotic Strain of Bean Common Mosaic Virus in Ethiopia

Abstract

Symptoms of the necrotic strain of bean common mosaic virus (BCMV) were observed on haricot bean (Phaseolus vulgaris) cv ‘Nicaragua 35’ during the 1995 cropping season in the international bean anthracnose trial at Ambo. The virus isolate showed a positive reaction on selected differential bean varieties. Further confirmation was made by ELISA, with monoclonal antibodies to Serotype A. The systemic necrotic reaction was localised-restricted veinal necrosis or black root and death of plants, depending on the differential variety used. When bean varieties with I-gene and non-I-gene were inoculated with the isolate they showed necrotic and mosaic symptoms, respectively, indicating that the isolate represents serotype A. This is the first confirmed record for the necrotic strain (serotype A) of BCMV in Ethiopia