In-vitro Evaluation of Indigenous Trichoderma Species for Bio-control of Faba Bean Root Rot Caused by Fusarium solani

Abstract

Fight Trichoderma species viz., T. oblongispontm, T. longibrachiatitm, T. asperi/lum, T. cerinum (tomentosum) T. harzianum, T. atroviride. T. viride and T. hamatum (seven of which were identified at molecular level) were isolated following standard procedure
from soil samples taken in different parts of Ethiopia and evaluated in-vitro for their antagonistic potential against root rot of faba bean caused by Fusarium so/ani. Dual culture technique was used for the investigations in which a 6mm diameter mycelial disc
of each o f the antagonists and the pathogen were confronted on opposite sides of 9cm Petri dishes. Radial growth o f the pathogen and the antagonist were recorded. All but one isolate was highly antagonistic that they covered and colonized the colony of the
pathogen. There was statistically significant difference (P=0.0005) among Trichoderma isolates in inhibiting the pathogen colony. The overall inhibition effect of the antagonists on the pathogen’s colony ranged from none to 50.5 %. Isolate of T. asperilhtm had the
highest inhibition effect (50.5%) followed by T. harzianum (48.1 %), T. hamatum (47.7%). T. viride (46.3 %) and T. longibrachiatum (43.6%) which were not significantly different one from each other. Therefore, these local isolates could be further studied in
the glasshouse (greenhouse) and field experiments and used in the biological control of the disease as one component of the integrated root rot management.